Role of metal ions on the secondary and quaternary structure of alkaline phosphatase from bovine intestinal mucosa

1999 ◽  
Vol 37 (2) ◽  
pp. 310-318 ◽  
Author(s):  
Muriel Bortolato ◽  
Françoise Besson ◽  
Bernard Roux
Keyword(s):  
Alkaline Phosphatase ◽  
Metal Ions ◽  
Intestinal Mucosa ◽  
Quaternary Structure

THE ROLE OF ALKALINE PHOSPHATASE IN INTESTINAL ABSORPTION: IV. THE EFFECTS OF VARIOUS PROTEINS ON LEVELS OF THE ENZYME IN INTESTINAL MUCOSA

1955 ◽  
Vol 33 (1) ◽  
pp. 89-92 ◽  
Author(s):  
Jules Tuba ◽  
Nester Dickie
Keyword(s):  
Alkaline Phosphatase ◽  
Intestinal Absorption ◽  
Intestinal Mucosa ◽  
Adult Male ◽  
Wheat Gluten ◽  
Male Rats ◽  
Dietary Proteins ◽  
Intestinal Alkaline Phosphatase ◽  
Egg Albumin

Fasted adult male rats were used to study the effect of dietary proteins on intestinal alkaline phosphatase. Groups of animals were offered one of several proteins; lactalbumin, egg albumin, zein, gelatin, wheat gluten, casein, and vitellin. Control animals had cellulose fed to them. The rats were sacrificed six hours after they were given the different diets. Alkaline phosphatase determinations with intestinal homogenates indicated that the two phosphoproteins, casein and vitellin, elevated levels of the enzyme significantly above fasting levels. Possible interpretations of these findings are discussed.

Role of Alkaline Phosphatase in Phosphate Uptake into Brush Border Membrane Vesicles from Human Intestinal Mucosa

1985 ◽  
Vol 97 (5) ◽  
pp. 1461-1466 ◽  
Author(s):  
Kazuyuki HIRANO ◽  
Yuichi IIIZUMI ◽  
Yukio MORI ◽  
Kazumi TOYOSHI ◽  
Mamoru SUGIURA ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Intestinal Mucosa ◽  
Brush Border ◽  
Brush Border Membrane ◽  
Phosphate Uptake ◽  
Membrane Vesicles ◽  
Brush Border Membrane Vesicles

THE ROLE OF ALKALINE PHOSPHATASE IN INTESTINAL ABSORPTION: II. THE EFFECTS OF VARIOUS CARBOHYDRATES ON LEVELS OF THE ENZYME IN INTESTINAL MUCOSA

1954 ◽  
Vol 32 (1) ◽  
pp. 621-624 ◽  
Author(s):  
Jules Tuba ◽  
Nestor Dickie
Keyword(s):  
Alkaline Phosphatase ◽  
Intestinal Absorption ◽  
Intestinal Mucosa ◽  
Adult Male ◽  
Enzyme Level ◽  
Male Rats ◽  
Significant Elevation ◽  
Intestinal Alkaline Phosphatase

Adult male rats were deprived of food for five days. Various groups of these animals were then offered cellulose or cellulose mixed with one of seven other carbohydrates. Six hours after food was placed before them, the animals were killed by decapitation, and a portion of the intestine from each rat was homogenized. Intestinal alkaline phosphatase estimations indicated (1) that the activity of the enzyme was increased significantly by the ingestion of glucose, galactose, fructose, and mannose, whereas (2) there was not a statistically significant elevation of the enzyme level in those animals which had been fed cellulose, arabinose, xylose, or sucrose. This would appear to suggest that alkaline phosphatase is involved in the intestinal absorption of some monosaccharides.

scholarly journals Kinetic Isotope Effects for Alkaline Phosphatase Reactions:  Implications for the Role of Active-Site Metal Ions in Catalysis

2007 ◽  
Vol 129 (31) ◽  
pp. 9789-9798 ◽  
Author(s):  
Jesse G. Zalatan ◽  
Irina Catrina ◽  
Rebecca Mitchell ◽  
Piotr K. Grzyska ◽  
Patrick J. O'Brien ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Metal Ions ◽  
Active Site ◽  
Isotope Effects ◽  
Kinetic Isotope Effects ◽  
Kinetic Isotope

scholarly journals Properties of inorganic pyrophosphatase of pig scapula cartilage

1975 ◽  
Vol 147 (1) ◽  
pp. 111-118 ◽  
Author(s):  
R Felix ◽  
H Fleisch
Keyword(s):  
Amino Acids ◽  
Molecular Weight ◽  
Alkaline Phosphatase ◽  
Metal Ions ◽  
Ammonium Molybdate ◽  
Inorganic Pyrophosphatase ◽  
Ph Optimum ◽  
Km Value ◽  
Calcification Process

The properties of a highly purified inorganic pyrophosphatase (pyrophosphate phosphohydrolase; EC 3.6.1.1) from pig scapula cartilage were studied. The enzyme had a molecular weight of 66 000 and a pH optimum of 7-8. It was markedly activated by magnesium, but not, or only to a much smaller degree, by other metal ions. PP1 was the only substrate found and had a Km value of 11 muM. The enzyme was not inhibited by phosphate and other inhibitors of alkaline phosphatase such as CN- minus, amino acids and theophylline; it was slightly inhibited by tartrate, formaldehyde and ammonium molybdate and strongly inhibited by F- minus, Ca2+ and other metal ions. The properties of the enzyme in the presence of concentrations of PP1 present in plasma (3.5 muM) were similar to those found at higher (2 mM) concentrations of PP1. The diphosphonates ethane-1-hydroxy-1,1-diphosphonate and dichloromethylenediphosphonate inhibited the enzyme in the presence of low PP1 concentrations. The characteristics of this enzyme are therefore similar to pyrophosphatases from other sources, such as from yeast and erythrocytes, and do not support a specific role of this enzyme in the calcification process.

THE ROLE OF ALKALINE PHOSPHATASE IN INTESTINAL ABSORPTION: IV. THE EFFECTS OF VARIOUS PROTEINS ON LEVELS OF THE ENZYME IN INTESTINAL MUCOSA

1955 ◽  
Vol 33 (1) ◽  
pp. 89-92 ◽  
Author(s):  
Jules Tuba ◽  
Nester Dickie
Keyword(s):  
Alkaline Phosphatase ◽  
Intestinal Absorption ◽  
Intestinal Mucosa ◽  
Adult Male ◽  
Wheat Gluten ◽  
Male Rats ◽  
Dietary Proteins ◽  
Intestinal Alkaline Phosphatase ◽  
Egg Albumin

Fasted adult male rats were used to study the effect of dietary proteins on intestinal alkaline phosphatase. Groups of animals were offered one of several proteins; lactalbumin, egg albumin, zein, gelatin, wheat gluten, casein, and vitellin. Control animals had cellulose fed to them. The rats were sacrificed six hours after they were given the different diets. Alkaline phosphatase determinations with intestinal homogenates indicated that the two phosphoproteins, casein and vitellin, elevated levels of the enzyme significantly above fasting levels. Possible interpretations of these findings are discussed.

scholarly journals Tryptophan phosphorescence as a monitor of the structural role of metal ions in alkaline phosphatase

1989 ◽  
Vol 185 (3) ◽  
pp. 573-579 ◽  
Author(s):  
Patrizia CIONI ◽  
Luciana PIRAS ◽  
Giovanni Battista STRAMBINI
Keyword(s):  
Alkaline Phosphatase ◽  
Metal Ions ◽  
Structural Role

THE ROLE OF ALKALINE PHOSPHATASE IN INTESTINAL ABSORPTION: II. THE EFFECTS OF VARIOUS CARBOHYDRATES ON LEVELS OF THE ENZYME IN INTESTINAL MUCOSA

1954 ◽  
Vol 32 (6) ◽  
pp. 621-624 ◽  
Author(s):  
Jules Tuba ◽  
Nestor Dickie
Keyword(s):  
Alkaline Phosphatase ◽  
Intestinal Absorption ◽  
Intestinal Mucosa ◽  
Adult Male ◽  
Enzyme Level ◽  
Male Rats ◽  
Significant Elevation ◽  
Intestinal Alkaline Phosphatase

Adult male rats were deprived of food for five days. Various groups of these animals were then offered cellulose or cellulose mixed with one of seven other carbohydrates. Six hours after food was placed before them, the animals were killed by decapitation, and a portion of the intestine from each rat was homogenized. Intestinal alkaline phosphatase estimations indicated (1) that the activity of the enzyme was increased significantly by the ingestion of glucose, galactose, fructose, and mannose, whereas (2) there was not a statistically significant elevation of the enzyme level in those animals which had been fed cellulose, arabinose, xylose, or sucrose. This would appear to suggest that alkaline phosphatase is involved in the intestinal absorption of some monosaccharides.

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